fast coomassie blue  (Elabscience Biotechnology)

Journal: Science Advances

Article Title: miRNA-loaded biomimetic nanoparticles orchestrate gut microbe to ameliorate inflammatory bowel disease

doi: 10.1126/sciadv.adw5984

Figure Lengend Snippet: ( A ) Representative size distribution of BEVs determined by NTA. ( B ) Representative zeta potential distribution of BEVs determined by electrophoretic light scattering. ( C ) Representative TEM image of BEVs after negative stain. Scale bar, 200 nm. ( D ) Hydrodynamic diameter of BEVs, LNPs, and BEV-LNPs. ( E ) Zeta potential of BEVs, LNPs, and BEV-LNPs. ( F ) Fluorescent spectra of LNPs after extrusion with BEVs at different ratios at the maximal excitation wavelength of DiI. DiI excitation wavelength (Ex): 550 nm, emission wavelength (Em): 565 nm; DiD Ex: 640 nm, Em: 670 nm. ( G ) Confocal laser scan microscope image of colocation of LNPs (green) and BEVs (red) after extrusion. Scale bar, 50 μm. ( H ) Representative TEM image of BEV-LNP. Scale bar, 100 nm. ( I ) SDS–polyacrylamide gel electrophoresis (SDS-PAGE) of proteins from BEVs, LNPs, and BEV-LNPs stained with Coomassie blue. ( J ) Volcano plot showing protein changes in BEV-LNPs and BEVs. The lateral dashed line denotes a threshold of P = 0.05, and the longitudinal dashed lines denote fold change values of 0.67 or 1.5. ( K ) Classification of BEV -LNP proteins by a cellular component. Typical GO terms related to the structure of the bacterial envelope were in rose-red color. ATP, adenosine triphosphate. ( L ) Classification of BEV-LNP proteins by a biological process. Typical GO terms related to biosynthetic process of the bacterial envelope were in green color, while typical GO terms related to uptake were in red color. ( M ) Classification of BEV-LNP proteins by molecular function. Typical GO terms related to homologous targeting were in red color. Data in (D) and (E) are represented as means ± SD ( n = 3 biologically independent samples).

Article Snippet: The gel was stained with Coomassie blue fast staining solution (Epizyme, China) without the decoloring procedure and visualized by a gel imaging system (ChemiDoc, Bio-Rad, USA).

Techniques: Zeta Potential Analyzer, Staining, Microscopy, Polyacrylamide Gel Electrophoresis, SDS Page